Corkscrew Swamp Sanctuary occupies approximately 13,000 acres in the heart of the Corkscrew Watershed in Southwest Florida, part of the Western Everglades. It is primarily composed of wetlands. These include the largest remaining virgin bald cypress forest in the world (approximately 700 acres), which is the site of the largest nesting colony of Federally Endangered Wood Storks in the nation.

In addition to the Wood Stork, Corkscrew provides important habitat for numerous other federal and state listed species, including the Florida Panther, American Alligator, Gopher Tortoise, Florida Sandhill Crane, Limpkin, Roseate Spoonbill, Snowy Egret, Tricolored Heron, White Ibis, Big Cypress Fox Squirrel, and the Florida Black Bear. Several rare plants are also found here, most notably the Ghost Orchid.